Study of endothelial dysfunction and asymmetric dimethylarginine levels

The aim of the study was to analyze changes in the level of endothelin-1 and asymmetric dimethylarginine in the development of endothelial dysfunction in experimental diabetic retinopathy and various methods of its correction.The study was performed on white Wistar rats weighing 180-200 g. According to the tasks of the animal were divided into 7 groups:As a result of our study proved a violation of the structural and functional state of the endothelium in experimental diabetic retinopathy, as evidenced by elevated levels of ADMA and endothelin-1 in 2nd group (p <0.001), most pronounced in the 3rd stage. It was confirmed that the correction of the studied complication of diabetes mellitus only with a hypoglycemic drug, even with long-term administration, does not correct the development of endothelial dysfunction (p <0.001).It was found that the addition of aflibercept and a solution of L-arginine in the correction to hypoglycemic drugs significantly (p <0.001) improves the condition of the endothelium, but does not solve the problem completely. It is observed that the correction of the simulated pathological condition by reducing hyperglycemia, administration of aflibercept and bromfenac (group № 5) has a positive effect on the normalization of endothelial function markers (p <0.001), but the effect is less pronounced than in the following groups. It was found that in rats in which diabetic retinopathy was simulated with subsequent correction of hyperglycemia, administration of aflibercept, L-carnitine and bromfenac (group № 6), the reduction of pathologically elevated levels of markers of endothelial dysfunction is more pronounced compared to the 3rd group, which indicates the feasibility of this method of correction. It was found that the most effective method of correction was in the 7th group of the experiment in which hyperglycemia was corrected, aflibercept, L-arginine and citicoline were obtained to normalize the levels of endothelial dysfunction markers - endothelin 1 and asymmetric dimethylarginine

Analysis interconnection level index marker by endothelial NOS, hypoxia and dysfunction in the pathogenesis of experimental diabetic retinopathy

The aim of the study was to analyze changes in erythrocyte diphosphoglycerate levels and Willebrand factor in the development of hypoxia and endothelial dysfunction in experimental diabetic retinopathy. The study was performed on white Wistar rats weighing 180-200 g. Our results indicate the development of hypoxia on the 30th day of development of experimental diabetic retinopathy with subsequent progression of pathological changes on the 60th and 180th day of the study, as evidenced by the decrease level of 2,3 diphosphoglycerate of erythrocytes in the 2nd group (p <0,001). The most pronounced increase in the studied marker of hypoxia was detected in the 3rd stage of the experiment (p <0,001). As a result of our study, a violation of the structural and functional state of the endothelium in experimental diabetic retinopathy was proved, as evidenced by an increase in the level of Willeband factor in group 2 (p <0.001), most pronounced in stage 3. The most pronounced increase in the level of Willebrand factor was detected in the 3rd stage of the experiment (p <0.001). Analyzing the data obtained, we can say that there is a relationship between the development of hypoxia and endothelial dysfunction in the pathogenesis of experimental diabetic retinopathy

Analysis of peroxidase activity in diabetic retinopathy and in applying various corrective means

Hyperglycemia stimulates the development of oxidative stress, which in turn is a powerful pathophysiological mechanism for the development of microvascular complications in diabetes. Increased production of reactive oxygen species is observed both during development and during the progression of diabetic retinopathy.The study was performed on white Wistar rats weighing 180-200 g. According to the tasks, the animals were divided into 7 groups: 1st group - 60 intact animals; Group 2 - 60 animals in which diabetic retinopathy was simulated without further correction. Group 3 - 60 animals, which simulated diabetic retinopathy with subsequent correction of hyperglycemia; Group 4 - 60 animals in which diabetic retinopathy was simulated with subsequent correction of hyperglycemia, administration of aflibercept and L-arginine solution; Group 5 - 60 animals in which diabetic retinopathy was simulated with subsequent correction of hyperglycemia, administration of aflibercept and bromfenac; Group 6 - 60 animals in which diabetic retinopathy was simulated with subsequent correction of hyperglycemia, administration of aflibercept, L-carnitine and bromfenac; Group 7 - 60 animals, which simulated diabetic retinopathy with subsequent correction of hyperglycemia, the introduction of aflibercept, a solution of L-arginine and citicoline.The results indicate the development of oxidative stress from the 30th and with subsequent progression on the 60th and 180th days of experimental diabetic retinopathy, which is confirmed by a decrease in peroxidase activity in the 2nd group, the maximum of which is observed in the 3rd stage. Correction with hypoglycemic agents in group 3 had a positive effect, but was not able to restore the activity of the antioxidant enzyme, so there was a need for additional drugs. The use of aflibercept and nitric oxide donor in group 4 to correct the development of diabetic retinopathy had a positive effect on increasing the activity of peroxidase, which peaked on the 180th day of the experiment, but did not reach the control values. The combined administration of bromfenac and aflibercept in group 5 was shown to significantly increase antioxidant activity, but not as significantly as in group 4. Administration of aflibercept, L-carnitine, and bromfenac to group 6 animals was shown to restore antioxidant protection as early as day 30 and was continued on days 60 and 180 of the study, but the results did not reach control values. The combination of metformin, aflibercept, L-arginine and citicoline in rats of the 7th group proved to be the most effective correction, as evidenced by the normalization of peroxidase activity on the 30th and 60th day of the experiment, and on the 180th recovery of marker activity to control values was recorded

Study of vasodilation processes

To date, the key role of endothelial dysfunction in the occurrence and progression of diabetes mellitus and diabetic retinopathy has been proven. The study was performed on white Wistar rats weighing 180-200 g. According to the tasks, the animals were divided into 7 groups. Our results indicate a violation of vasodilation on the 30th day of experimental diabetic retinopathy with subsequent progression of pathological changes on the 60th and 180th day of the study, as evidenced by a decrease in the content of S-nitrosothiols in group 2 (p <0,001), most pronounced in the 3rd stage. When analyzing the data of group № 3, it was found that the correction of the pathological condition with the help of hypoglycemic agents has some positive effect, but does not allow to significantly adjust the pathological development of reduced vasodilatory potential. The results of the 4th group indicate that the involvement of nitric oxide donor and aflibercept in the correction of diabetic retinopathy corrects the pathological changes and helps to restore the physiological pathway of nitric oxide synthesis and vascular tone, the maximum effect is observed on the 180th day of the experiment, but normative cannot be achieved. It is observed that the correction of the simulated pathological condition by reducing hyperglycemia, administration of aflibercept and bromfenac (group № 5) gives positive results in the first stage, but less pronounced than involvement in the complex correction of L-arginine solution in subsequent stages. It was found that rats in which diabetic retinopathy was simulated with subsequent correction of hyperglycemia, administration of aflibercept, L-carnitine and bromfenac (group № 6) have a pronounced tendency to normalize the studied marker of hypoxia in comparison with the previous methods in the second stage. nitrosothiols at the time of reaching the 3rd stage is reduced. The obtained data suggest that the method of correction chosen in group 7 (correction of hyperglycemia, aflibercept, L-arginine and citicoline solution) more pronouncedly normalizes the content of the vasodilation marker compared to other groups of our experiment, which is pronounced in long-term correction - on 180th day

Features of the system of antioxidant protection and lipid peroxidation in microangiopathies against type 2 diabetes mellitus

The article presents the results of an experimental study of the AOР and LPО system in animals simulated complications of the microcirculatory tract (diabetic retinopathy, diabetic nephropathy) on the background of type 2 diabetes. It was found that in the group of animals with type 2 diabetes increased the level of DC, TBCproducts and MDA; catalase and FG levels decreased. In animals with simulated DN, AOP disorders were more pronounced compared to animals with DR. This trend can be explained by the fact that in addition to hyperglycemia in these animals, the excessive formation of free radicals contributes to metabolic shifts in ischemic areas of tissues and organs. Disturbance of balance in AOP and LPO in type 2 diabetes triggers a pathogenetic cascade of development of complications of the microcirculatory tract and is accompanied by a tendency to further increase the generation of reactive oxygen species and activation of POL in microangiopathies

Justification of the renoprotective action of the mixture of sodium chloride and sodium bicarbonate solutions in phenylhydrazine intoxication

The authors conducted a study on 35 white Wistar outbred rats to investigate the possibility of correcting acute kidney damage induced by the administration of phenylhydrazine at a dose of 100 mg/kg by introducing a mixture of sodium chloride and sodium bicarbonate solutions into the body. The research results identified changes in the kidneys upon phenylhydrazine administration, including the loss of some capillary glomeruli, eosinophilic deposits in Bowman's spaces and tubular lumens, and lymphoid infiltration in the interstitium. Rats receiving a mixture of sodium chloride and sodium bicarbonate solutions in their drinking water showed positive changes in their kidneys: no loss of capillary glomeruli was observed, and eosinophilic deposits were absent in most tubules. Lymphocyte aggregation was only observed around some renal vessels. The authors suggest that the intake of additional sodium and bicarbonate into the body, along with alkalinization of the primary urine, promotes the excretion of hemolysis products caused by phenylhydrazine, which contributes to renoprotection and preservation of renal parenchyma

Функціональний стан нирок після введення клітин фетальної печінки щурам з водним і сольовим режимом пиття за умов розвитку гострої сулемової нефропатії

The experiments on non linear white rats with acute sublimate nephropathy showed that in 7 days after intraperitoneal injection of fetal liver cells (FLC ) of rats plasma creatinine concentration decreased and proximal reabsorption of sodium ions increased independently of water or salt drinking regime. The increase of natremia in rats that used tap water excluded regulatory influence of FLC on the processes of maintenance of sodium balance under the conditions of experiment. Usage of 0.9 % NaCl solution leads to increase of glomerular filtration rate, activation of tubular transport of sodium ions with predominant elevation of reabsorption in the proximal part of nephron, reduction of sodium ions loss with urination. That points to substantial role of salt drinking regimen in additional beneficial effects of FLC on renal processes in rats with acute sublimate nephropathy.В экспериментах на нелинейных белых крысах с острой сулемовой нефропатией установлено, что через 7 дней после внутрибрюшного введения клеток фетальной печени (КФП) крысам уменьшалась концентрация креатинина в плазме крови и увеличивалась проксимальная реабсорбция ионов натрия независимо от водного или солевого режима питья. Повышение уровня натриемии у животных, употреблявших водопроводную воду, исключало регуляторное влияние КФП на процессы обеспечения натриевого баланса в этих условиях эксперимента. При употреблении 0,9 % раствора NaCl увеличивалась скорость клубочковой фильтрации, активировался канальцевый транспорт ионов натрия с преимущественным повышением реабсорбции в проксимальном отделе нефрона, уменьшалась потеря ионов натрия с мочой, что указывает на существенную роль солевого режима питья для дополнительного благоприятноговоздействия КФП на почечные процессы у крыс с острой сулемовой нефропатией.В експериментах на нелінійних білих щурах із гострою сулемовою нефропатією встановлено, що через 7 днів після внутрішньочеревного введення клітин фетальної печінки (КФП) щурам зменшувалась концентрація креатиніну в плазмі крові та збільшувалась проксимальна реабсорбція іонів натрію незалежно від водного чи сольового режиму пиття. Підвищення рівня натріємії у тварин, які вживали водопровіднуводу, виключало регуляторний вплив КФП на процеси забезпечення натрієвого балансу в організмі за цихумов експерименту. При вживанні 0,9 % розчину NaCl збільшувалась швидкість клубочкової фільтрації,активувався канальцевий транспорт іонів натрію з переважним підвищенням реабсорбції в проксимальному відділі нефрону, зменшувалась втрата іонів натрію із сечею, що вказує на значну роль сольового режиму пиття для додаткового сприятливого впливу КФП на ниркові процеси у щурів із гострою сулемовою нефропатією

Гостре ураження нирок при запальних процесах і шляхи їх корекції = Acute kidney damage in inflammatory processes and ways of their correction

Sirman V. M., Boris R. M., Nykytenko O. P., Zukow W., Gozhenko A. I. Гостре ураження нирок при запальних процесах і шляхи їх корекції = Acute kidney damage in inflammatory processes and ways of their correction. Journal of Education, Health and Sport. 2016;6(1):349-368. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.47347http://ojs.ukw.edu.pl/index.php/johs/article/view/3417https://pbn.nauka.gov.pl/works/719461  The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015).755 Journal of Education, Health and Sport eISSN 2391-8306 7© The Author (s) 2016; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, PolandOpen Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License(http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercialuse, distribution and reproduction in any medium, provided the work is properly cited.The authors declare that there is no conflict of interests regarding the publication of this paper.Received: 01.01.2016. Revised 12.01.2016. Accepted: 31.01.2016.   УДК 616.61-002 Гостре ураження нирок при запальних процесах і шляхи їх корекції В. М. Сірман, Р. М. Борис, О. П. Никитенко, В. A. Жуков, А. І. Гоженко Державне підприємство «Український науково-дослідний інститут медицини транспорту МОЗ України», м. Одеса, УкраинаUniwersytet Kazimierza Wielkiego, Bydgoszcz, Polska РезюмеМетою дослідження було вивчення функціонального стану та механізмів порушеннями нирок при експериментальному перитоніті, а також при ад’ювантному артриті Пірсона у щурів. Для вирішення поставлених задач проведені серії експериментів in vivo. У роботі використано 189 самців білих щурів з середньою масою тіла 0,193±0,018 кг. Дослідження проводили через 2, 4, 6 і 12 міс. після індукції ад’ювантного артриту Пірсона.  При експериментальному перитоніті, проведено 7 серій експериментів на 486 самцях білих щурів з масою тіла 0,17-0,30 кг. Встановлено, що при обох експериментальних моделях запалення виникають однотипні порушення функції нирок, які виявляються у зменшенні діурезу, зростанні екскреції білка та натрію, що пов’язано як із падінням швидкості клубочкової фільтрації, так і канальцевої реабсорбції води та натрію. Їх розвиток відрізняється у часі та залежить від строків виникнення та динаміки запального процесу. Ключові слова: гостре ураження нирок, ХХН, ХНН, ШКФ. Acute kidney damage in inflammatory processes and ways of their correction V. M. Sirman, R. M. Boris, O. P. Nykytenko, W. Zukow, A. I. Gozhenko SE "Ukrainian Scientific Research Institute of Transport Medicine, Ministry of Health of UkraineKazimierz Wielki University, Bydgoszcz, Poland AbstractThe aim of the study was to investigate functional status and mechanisms of disorders of the kidneys in experimental peritonitis, as well as adjuvant arthritis in rats Pearson. To solve this problem, a series of experiments in vivo. The paper used 189 male albino rats with an average body weight of 0,193 ± 0,018 kg. The study was conducted at 2, 4, 6 and 12 months. After induction of adjuvant arthritis Pearson. In experimental peritonitis, 7 held a series of experiments on 486 white male rats weighing 0,17-0,30 kg. Found that in both experimental models of inflammation arising same type of renal dysfunction, which are to reduce urine output, increasing excretion of sodium and protein, which is associated with a drop in glomerular filtration rate and tubular reabsorption of water and sodium. Their development is different in time and depends on the timing of the emergence and dynamics of inflammation. Keywords: acute kidney damage, CKD, chronic renal failure, GFR

Вплив трансплантації ембріональних прогеніторних клітин на функцію нирок = Effect of transplantation embryonic progenitor cells on renal function

Sirman V. M., Borys R. M., Nykytenko O. P., Zukow W., Gozhenko A. I. Вплив трансплантації ембріональних прогеніторних клітин на функцію нирок = Effect of transplantation embryonic progenitor cells on renal function. Journal of Education, Health and Sport. 2016;6(2):349-366. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.56017http://ojs.ukw.edu.pl/index.php/johs/article/view/3614 The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015).755 Journal of Education, Health and Sport eISSN 2391-8306 7© The Author (s) 2016;This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, PolandOpen Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License(http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercialuse, distribution and reproduction in any medium, provided the work is properly cited.The authors declare that there is no conflict of interests regarding the publication of this paper.Received: 05.01.2016. Revised 12.02.2016. Accepted: 27.02.2016. УДК: 616.61-008-089.843:615.361.013  В. М. Сірман, Р. М. Борис, О. П. Никитенко, В. Жуков, А. І. Гоженко Український науково-дослідний інститутмедицини транспорту МОЗ України, м. Одеса, УкраинаUMK w ToruniuРезюмеМетою нашого дослідження було вивчення  впливу трансплантації ембріональних прогеніторних клітин на функцію нирок при експериментальному перитоніті та при ад’ювантному артриті Пірсона у щурів. У роботі використано 189 самців білих щурів з середньою масою тіла 0,193±0,018 кг. Дослідження проводили через 2, 4, 6 і 12 міс. після індукції ад’ювантного артриту Пірсона.  При експериментальному перитоніті, проведено 7 серій експериментів на 486 самцях білих щурів з масою тіла 0,17-0,30 кг. Встановлено, що при обох експериментальних моделях запалення трансплантація ембріональних прогеніторних клітин сприяє значному підвищенню діурезу за рахунок збільшення швидкості клубочкової фільтрації, що суттєво знижує вміст креатиніну у плазмі крові. Крім того, під впливом трансплантації ембріональних прогеніторних клітин спостерігається дворазове зниження втрат білка з кінцевою сечею, стандартизованих за об’ємом клубочкового фільтрату. Ключові слова: гостре ураження нирок, ШКФ, трансплантація ембріональних прогеніторних клітин.   EFFECT OF TRANSPLANTATION EMBRYONIC PROGENITOR CELLS ON RENAL FUNCTION V. M. Sirman, R. M. Borys, O. P. Nykytenko, W. Zukow, A. I. Gozhenko Ukrainian Scientific Research Institute of Transport Medicine, Ministry of Health of UkraineUMK w ToruniuResumeThe aim of our study was to examine the influence of transplantation of embryonic progenitor cells in renal function in experimental peritonitis and in adjuvant arthritis in rats Pearson. The paper used 189 male albino rats with an average body weight of 0,193 ± 0,018 kg. The study was conducted at 2, 4, 6 and 12 months. After induction of adjuvant arthritis Pearson. In experimental peritonitis, held 7 series of experiments on 486 white male rats weighing 0,17-0,30 kg. Found that in both experimental models of inflammation transplantation of embryonic progenitor cells contributes to a significant increase in urine output by increasing glomerular filtration rate, which significantly reduces creatinine in blood plasma. In addition, under the influence of transplantation of embryonic progenitor cells there is a twofold decrease loss of protein from the final urine volume standardized glomerular filtrate. Keywords: acute kidney damage, GFR, Transplantation of embryonic progenitor cells

Key Agent and Survivor Recommendations for Intervention in Honour-Based Violence in the UK

This paper concerns recommendations for intervention in honour-based violence (“HBV”) as recommended by individuals who face such violence in their everyday lives. Utilising data extracted from interviews conducted with 30 key agents and 8 South-Asian female survivors in the UK, this paper will argue that UK public agencies are struggling to cope with how to respond to HBV. This is despite the UK government recognising shortcomings in the support for victims in the House of Commons Home Affairs Committee report in 2008. In particular, participants identified that (a) the police, healthcare, and social services are particularly poor at supporting victims; (b) public sector workers require appropriate training and awareness on HBV; (c) education on HBV and forced marriages is absent in schools, colleges, and universities; and (d) more needs to be done to engage and educate communities about HBV and where victims can access support